Tasca S, Carli E, Caldin M, Menegazzo L, Furlanello T, Gallego LS.
Hematologic abnormalities and flow cytometric immunophenotyping results in dogs with hematopoietic neoplasia: 210 cases (2002-2006)
Vet Clin Pathol. 2009 Mar;38(1):2-12. doi: 10.1111/j.1939-165X.2008.00099.x.
Abstract
BACKGROUND: Growing interest in veterinary oncohematology has facilitated the recent development and advancement of new techniques, such as flow cytometry, for immunophenotyping hematopoietic neoplasia in animals.
OBJECTIVE: The aim of this retrospective study was to characterize hematologic abnormalities and flow cytometric immunophenotyping (FCI) results in cases of hematopoietic neoplasia in dogs.
METHODS: Signalment, CBC data, and FCI results were obtained for 210 dogs with blood samples submitted to our laboratory. Immunophenotyping was carried out using an Epics XL-MCL flow cytometer and a panel of 10 antibodies (CD45, CD3, CD4, CD8, CD79, CD21, CD14, CD34, CD41/61, CD61). The prevalence and severity of hematologic abnormalities was determined for the different types of hematopoietic neoplasms.
RESULTS: Based on cell morphology and phenotype, cases were classified as: acute lymphoblastic leukemia (ALL, n=51), acute myeloid leukemia (AML, n=33), chronic lymphocytic leukemia (CLL, n=61), and leukemic high-grade lymphoma (L-HGL, n=65). Most cases of ALL (47/51) and L-HGL (41/65) had a B-cell phenotype, while most cases of CLL (54/61) had a T-cell phenotype, with a high prevalence of the large granular lymphocyte subtype (49/61). Anemia was found in 85% of all cases and was significantly more severe in ALL and AML compared with CLL and L-HGL. Neutropenia was seen in 64-78% of acute leukemias (AML and ALL) in contrast to no cases of CLL and 11% of L-HGL. Thrombocytopenia was seen in 88-90% of acute leukemias in contrast to 15% of CLL and 40% of L-HGL. Thrombocytopenia was more prevalent (71% vs 22%) and significantly more severe in T-cell vs B-cell L-HGL.
CONCLUSION: A standard CBC is useful in suggesting the type of hemoproliferative disorder and may also help to predict the phenotype, especially in cases of L-HGL.
La Clinica ed il Laboratorio San Marco rientrano tra le attività di impresa di pubblica utilità, per le quali il DPCM non determina il blocco delle attività produttive, delle attività lavorative. Applichiamo con rigore tutte le misure di legge e le raccomandazioni per limitare la diffusione dell’infezione ed una serie di ulteriori misure interne, per la salvaguardia della nostra e della vostra salute. E siamo certi della vostra comprensione e collaborazione.
La clinica invia a tutti i clienti con appuntamento un documento della Direzione Sanitaria che certifica la necessità di recarsi in Clinica per le cure al vostre pet o per ritirare il vostro pet alle dimissioni. Potete comunque richiederlo a reception@sanmarcovet.it (o tramite messaggio whats app che trovate in homepage). Portate con voi la mascherina!
Il Direttore Sanitario Dott. Marco Caldin